Immunomodulatory effect of granulocyte colony-stimulating factor in repeated implantation failures in embryo transfer programs

The objective: to determine the effectiveness of intrauterine administration granulocyte colony-stimulating factor depending on endometrium immune phenotype in patients with repeated implantation failures embryo transfer programs.Material and methods. Endometrial biopsy during window an artificial cycle before (in some patients) after (G-CSF) was performed 42 gestational carriers (20 women) as a control group. Flow cytometry used count classes subclasses endometrial lymphocytes tissue samples.Results. population cells fertile women compared. Pregnancy live birth rates program were determined effect evaluated.High expression HLA-DR CD16 uterine natural killers is reliably associated successful program. It established that group immature frequency pregnancy (53.8 %) births twice higher compared rest other variants or absence changes profile (pregnancy rate – 26.9 %).Conclusions. One-third genetically tested embryos programs have unique status characterized by high killers. Intrauterine leads decrease promotes this patients.